Laurentiu Spiridon, Dr.

Laurentiu Spiridon
Group: Bioinformatics & Structural Biochemistry
Department: Bioinformatics & Structural Biochemistry


Laurentiu Spiridon is a researcher in the Institute of Biochemistry of the Romanian Academy. Laurentiu is currently working in Bioinformatics & Structural Biochemistry in the Bioinformatics & Structural Biochemistry.


  • Chiritoiu G., Munteanu CVA., Sulea TA., Spiridon L., Petrescu AJ., Jandus C., Romero P., Petrescu SM.Chiritoiu G. et al . "Methionine oxidation selectively enhances T cell reactivity against a melanoma antigen", iScience(107205), (2023)
    doi: 10.1016/j.isci.2023.107205
    IF: 6.10AI: 1.63
  • Martin EC, Ion CF, Ifrimescu F, Spiridon L, Bakker J, Goverse A, Petrescu AJ.Martin EC et al . "NLRscape: an atlas of plant NLR proteins", Nucleic Acids Res., (2022)
    IF: 19.16AI: 5.51
  • Manoliu LCE, Martin EC, Milac AL, Spiridon LManoliu LCE et al . "Effective Use of Empirical Data for Virtual Screening against APJR GPCR Receptor", Molecules (Basel, Switzerland) 26(16), (2021)
    IF: 4.41AI: 0.60
  • Spiridon L, Şulea TA, Minh DDL, Petrescu AJ.Spiridon L et al . "Robosample: A Rigid-Body Molecular Simulation Program Based on Robot Mechanics", Biochimica Biophysica Acta General Subjects 1864(8): 129616, (2020)
    IF: 3.68AI: 1.40
  • Martin EC, Sukarta OCA, Spiridon L, Grigore LG, Constantinescu V, Tacutu R, Goverse A, Petrescu AJMartin EC et al . "LRRpredictor-A New LRR Motif Detection Method for Irregular Motifs of Plant NLR Proteins Using an Ensemble of Classifiers", Genes (Basel) 11(3): 286, (2020)
    IF: 3.33
  • Spiridon L, Minh DDL. "Hamiltonian Monte Carlo with Constrained Molecular Dynamics as Gibbs Sampling", Journal of chemical theory and computation 13(10): 4649-4659, (2017)
    IF: 5.40
  • Sueldo DJ, Shimels M, Spiridon LN, Caldararu O, Petrescu AJ, Joosten MH, Tameling WI. et alSueldo DJ et al . "Random Mutagenesis of the Nucleotide-Binding Domain of NRC1 (NB-LRR Required for Hypersensitive Response-Associated Cell Death-1), a Downstream Signalling Nucleotide-Binding, Leucine-Rich Repeat (NB-LRR) Protein, Identifies Gain-Of-Function Mutations in the Nucleotide-Binding Pocket", New Phytologist 208(1): 210-223, (2015)
    IF: 7.43AI: 2.50
  • Sela H, Spiridon LN, Ashkenazi H, Bhullar NK, Brunner S, Petrescu AJ, Fahima T, Keller B, Jordan T.Sela H et al . "Three-dimensional Modeling and Diversity Analysis Reveals Distinct AVR Recognition Sites and Evolutionary Pathways in Wild and Domesticated Wheat Pm3 R Genes", Molecular Plant Microbe Interactions 27(8): 835-845, (2014)
    IF: 3.94AI: 1.30
  • Ciubotaru M, Trexler AJ, Spiridon LN, Surleac MD, Rhoades E, Petrescu AJ, Schatz DG.Ciubotaru M et al . "RAG and HMGB1 create a large bend in the 23RSS in the V(D)J recombination synaptic complexes", Nucleic Acids Research 41(4): 2437-2454, (2013)
    IF: 8.81AI: 3.40
  • Slootweg EJ, Spiridon LN, Roosien J, Butterbach P, Pomp R, Westerhof L, Wilbers R, Bakker E, Bakker J, Petrescu AJ, Smant G, Goverse A.Slootweg EJ et al . "Structural determinants at the interface of the ARC2 and leucine-rich repeat domains control the activation of the plant immune receptors Rx1 and Gpa2", Plant Physiology 162(3): 1510‐1528, (2013)
    IF: 7.39AI: 2.40
  • Sela H, Spiridon LN, Petrescu AJ, Akerman M, Mandel-Gutfreund Y, Nevo E, Loutre C, Keller B, Schulman AH, Fahima T.Sela H et al . "Ancient Diversity of Splicing Motifs and Protein Surfaces in the Wild Emmer Wheat (Triticum Dicoccoides) LR10 Coiled Coil (CC) and Leucine-Rich Repeat (LRR) Domains", Molecular Plant Pathology 13(3): 276‐287, (2012)
    IF: 4.49AI: 1.30
  • Maekawa T, Cheng W, Spiridon LN, Töller A, Lukasik E, Saijo Y, Liu P, Shen QH, Micluta MA, Somssich IE, Takken FLW, Petrescu AJ, Chai J, Schulze-Lefert PMaekawa T et al . "Coiled-coil domain-dependent homodimerization of intracellular barley immune receptors defines a minimal functional module for triggering cell death", Cell Host Microbe 9(3): 187‐199, (2011)
    IF: 13.50AI: 7.00
  • Slootweg E, Roosien J, Spiridon LN, Petrescu AJ, Tameling W, Joosten M, Pomp R, van Schaik C, Dees R, Borst JW, Smant G, Schots A, Bakker J, Goverse A.Slootweg E et al . "Nucleocytoplasmic Distribution Is Required for Activation of Resistance by the Potato NB-LRR Receptor Rx1 and Is Balanced by Its Functional Domains", Plant Cell 22(12): 4195‐4215, (2010)
    IF: 9.40AI: 4.00
  • Surleac MD, Spiridon LN, TacutuR, Milac AL, Petrescu SM, Petrescu AJSurleac MD et al . "Structural Assessment of Glycosylation Sites Database - SAGS – An Overall View on N-Glycosylation", pp 3-20, Glycosilation, InTech, (2012).
    ISBN: 978-953-51-0771-2
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Gerontomics: Multi-omics prediction system for prioritization of gerontological interventions 2016-2021
Acronym: Gerontomics
Project director: Robi Tacutu

Starting 02.09.2016, the Institute of Biochemistry of the Romanian Academy is implementing the project “Multi-omics prediction system for prioritization of gerontological interventions”, co-funded through European Fund for Regional Development, in accordance with the funding contract signed by the Ministry of National Education and Scientific Research. The total funding for the project is 8.524.757,50 lei, of which 8.502.557,50 lei represent non-reimbursable funding. The project’s duration is 48 months.

High-throughput screening platform for small-molecules with anti-inflammatory potential 2020-2022
Acronym: HTS-IL-1β
Budget: 600.000 RON
Project director: Marioara Chiritoiu-Butnaru

This project aims to develop a sensitive high-throughput screening platform by generating an endogenously tagged interleukin-1β reporter cell line by CRISPR-Cas9 technology, able to monitor stimulated IL-1β secretion with the purpose to identify new chemical compounds with anti-inflammatory activity that will be validated in primary macrophages and a mouse model for sepsis.

Free energy prediction of biomolecular processes using high speed robotics algorithms and its use in in-silico driven experimental research 2018-2020
Acronym: ROBOFEP
Project director: Laurentiu Spiridon

By developing new methods which leverage high-speed robotics algorithms, we undertake the effort to enhance free energy prediction for biomolecular processes. Our focus includes integrating these advancements into existing APIs and applying them to key areas of molecular biosciences such as plant-pathogen interaction, drug design, and immunotherapy.

Exploitation of innovative targets against Alzheimer's disease and related neurodegenerative disorders 2018-2021
Acronym: XploitAD
Project director: Laurentiu Spiridon

This project aims to enhance our understanding of the complex biochemical processes involved in Alzheimer's disease and overcome existing limitations in amyloid research by developing an analytical system capable of detecting amyloid concentration during the aggregation stage and advancing drug development in this field.

Usage of high speed robotics algorithms for in-sillico assisted experimental research of large biomolecular systems 2021–2023
Acronym: CYBORG
Project director: Laurentiu Spiridon

This project aims to improve protein engineering for medical and food security applications by leveraging advanced in silico assistance. Our software, Robosample, combines robotics algorithms with Gibbs sampling to accurately recover biomolecular free energy surfaces. We will further integrate robotics and molecular simulation by introducing new non-equilibrium sampling methods and novel robotic joints.