The central research topic of the group is the study of the structure-function relationships of signaling enzymes, with emphasis on tyrosine phosphatase proteins. We aim to contribute to the understanding of how their structural characteristics are correlated with specific signaling functions. To this end, we analyze each signaling enzyme that we investigate from several directions:
The combination of results thus obtained in this way is further used to shed light on the signaling mechanism and the overall functional role of the given enzyme.
The group has good experience and is currently involved in the production, isolation and purification of recombinant proteins, expressed in both prokaryotic and eukaryotic systems. The research activity of the group is performed by molecular biology instruments (recombinant DNA, site-directed mutagenesis, (RT) -PCR, Western blot, immunoprecipitation, etc.), spectroscopic analysis (UV-VIS and fluorescence spectrophotometry), cell biology, protein crystallization and enzyme kinetic analysis.
Our team is involved in numerous national research projects and international collaborations.
Thus, the specific research projects in progress are: