Stefan Szedlacsek, Dr.
Currently working on
Department of Enzymology .
. "Synthesis and characterization of a novel [52Mn]Mn-labelled affibody based radiotracer for HER2+ targeting", Inorganic Chemistry Frontiers, (2023)
IF: 7.78AI: 1.01
. "Designed Peptide Inhibitors of STEP Phosphatase-GluA2 AMPA Receptor Interaction Enhance the Cognitive Performance in Rats", JOURNAL OF MEDICINAL CHEMISTRY 1520-4804(ISSN 0022-2623): 217–233, (2022)
IF: 7.45AI: 1.58
. "Trojan horse treatment based on PEG-coated extracellular vesicles to deliver doxorubicin to melanoma in vitro and in vivo", Cancer biology & therapy 23(1): 1-16, (2022)
. "Regulation of TRPM8 channel activity by Src-mediated tyrosine phosphorylation", Journal of Cellular Physiology 235(6): 5192-5203, (2020)
IF: 4.52AI: 1.50
. "Biological and molecular modifications induced by cadmium and arsenic during breast and prostate cancer development", Environ Res 178: 108700, (2019)
. "Analysis of EYA3 Phosphorylation by Src Kinase Identifies Residues Involved in Cell Proliferation", International Journal of Molecular Sciences 20(24): 6307, (2019)
. "Crystal structure of a xylulose 5-phosphate phosphoketolase. Insights into the substrate specificity for xylulose 5-phosphate", Journal of Structural Biology 207(1): 85-102, (2019)
. "Collagen regulates the ability of endothelial progenitor cells to protect hypoxic myocardium through a mechanism involving miR-377/VE-PTP axis", Journal of cellular and molecular medicine 22(10): 4700-4708, (2018)
. "WDR1 is a novel EYA3 substrate and its dephosphorylation induces modifications of the cellular actin cytoskeleton", Scientific reports 8(1): 2910, (2018)
. "Expression, Purification, and Kinetic Analysis of PTP Domains", Methods in molecular biology (Clifton, N.J.) 1447: 39-66, (2016)
. "Phosphoketolases from Lactococcus lactis, Leuconostoc mesenteroides and Pseudomonas aeruginosa: Dissimilar sequences, similar substrates but distinct enzymatic characteristics", Applied Microbiology and Biotechnology 18(98): 7855-67, (2014)
. "Analysis of molecular determinants of PRL-3", Journal of cellular and molecular medicine 13(9B): 3141-50, (2009)
. "Expression, Purification, and Kinetic Analysis of PTP Domains", pp 39- 66, Protein Tyrosine Phosphatases, Rafael Pulido, (2016).
. "Time-Dependent or Steady-State Control of Metabolic Systems?", pp 251-258, Technological and Medical Implications of Metabolic Control Analysis, A.Cornish-Bowden and M.L.Cardenas, NATO Science Series, 3.High Technology, (2000).
. "Kinetics of slow and tight-binding inhibitors", pp 144-180, Fundamentals of Enzyme Kinetics, Revised edition by Athel Cornish-Bowden, ACADEMIC PRESS INC, 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495, (1995).
The main idea of this project was that by inhibiting at least one of these two interactions of GluA2, the internalization of AMPAR will be reduced and therefore the synapse resistance will be increased, thus leading to improved cognitive functions.
The project is agreed as a bilateral collaboration between IBAR and ATOMKI, and the University of Debrecen and IFIN-HH participate in this project voluntarily.
The project is agreed as a joint collaboration among IBAR, ATOMKI and UD, the latter being a cost free participant. There are two main directions envisaged by the proposed project: - receptors mapping and therapy, using an affibody against HER2 receptors, combined with an adequate radioisotope. In this respect, the specific objectives are: a) expression and purification of affibodies; b) establishing labelling procedures; c) ex vivo and/or in vivo testing of optimal compounds.
Affibody molecules are highly promising therapeutic candidates due to their advantageous features like: small size, efficient delivery, straightforward engineering towards improved formats, site-directed conjugation of payloads, possibility of GMP production by chemical synthesis or inexpensive bacterial production leading to low product costs.
Project Funded under: Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006. PTPNET is a training network for young scientists in the field of protein tyrosine phosphatase (PTP) research.
Proteins are essential players of all biological processes and they are involved in practically every function performed by a living cell.