Stefan Szedlacsek, Dr.

Stefan Szedlacsek
Group: Enzymology
Department: Enzymology

Head of Department

Research interests: TBA, Researcher | Teaching staff | Scientific reviewer | Manager | Consultant.

Currently working on

Institute of Biochemistry of the Romanian Academy , Bucharest.
Department of Enzymology .

Biography

Professor Dr. Stefan Eugen Szedlacsek is the Head of the Enzymology Department at the Institute of Biochemistry of the Romanian Academy. He holds a PhD degree in Biotechnology from Polytechnic University of Bucharest as well as a MSc in Organic Synthesis (Polytechnic University- Bucharest) and MSc in Mathematics (Bucharest University). As a visiting scientist, he performed research in the field of cholesterol metabolism University of Illinois at Urbana-Champaign (USA), where he succeeded to evidence a new pathway in the metabolism of oxysterols. He is an “Alexander von Humboldt“ fellow and worked in Germany, in the Institute of Biochemistry (Kiel University), Max-Plack Institute for Physiological Chemistry (Dortmund) and Max-Planck Institute for Biophysics (Frankfurt) where he solved the crystal structure of PTP-SL/PTPBR7 catalytic domain, the first structure of a KIM-containing Protein Tyrosine Phosphatases PTP), and demonstrated the lateral dimerization of extracellular region of receptor-like PTP μ. Currently, Professor Szedlacsek and his team studies structure-function relationship in enzymes involved in cellular signaling. He has supervised numerous PhD and master’s theses. Together with his group, he participated as Principal Investigator/Group Leader in the Marie Curie Research Training Networks - PTPNET; MRTN-CT- 2006-035830 (FP6), financed by the European Community. Also, he received a „P-CUBE TNA Project” Fellowship (FP 7- EU) from Oxford University (United Kingdom). Professor Szedlacsek was Project Coordinator for research consortia within a number of projects financed by the Romanian Research Authority.

Papers

Year
  • Szedlacsek HS, Bajusz D, Badea RA, Pop A, Bică CC, Ravasz L, Mittli D, Mátyás D, Necula-Petrăreanu G, Munteanu CVA, Papp I, Juhász G, Hritcu L, Keserű GM, Szedlacsek SE.Szedlacsek HS et al . "Designed Peptide Inhibitors of STEP Phosphatase-GluA2 AMPA Receptor Interaction Enhance the Cognitive Performance in Rats", JOURNAL OF MEDICINAL CHEMISTRY 1520-4804(ISSN 0022-2623), (2021)
    IF: 7.45
  • Patras L, Ionescu AE, Munteanu C, Hajdu R, Kosa A, Porfire A, Licarete E, Rauca VF, Sesarman A, Luput L, Bulzu P, Chiroi P, Tranca RA, Meszaros MS, Negrea G, Barbu-Tudoran L, Potara M, Szedlacsek S, Banciu MPatras L et al . "Trojan horse treatment based on PEG-coated extracellular vesicles to deliver doxorubicin to melanoma in vitro and in vivo", Cancer biology & therapy: 1-16, (2021)
    IF: 4.74
  • Alexandra Manolache, Tudor Selescu, G. Larisa Maier, Mihaela Mentel, Aura Elena Ionescu, Cristian Neacsu, Alexandru Babes, Stefan Eugen SzedlacsekAlexandra Manolache et al . "Regulation of TRPM8 channel activity by Src-mediated tyrosine phosphorylation", Journal of Cellular Physiology 235(6): 5192-5203, (2020)
    IF: 4.52AI: 1.50
  • Alina-Andreea Zimta, Vlad Schitcu, Eugen Gurzau, Crina Stavaru, Gina Manda, Szedlacsek SE, Ioana Berindan-NeagoeAlina-Andreea Zimta et al . "Biological and molecular modifications induced by cadmium and arsenic during breast and prostate cancer development", Environ Res 178: 108700, (2019)
    IF: 5.03
  • Ionescu AE, Mentel M, Munteanu CVA, Sima LE, Martin EC, Necula-Petrareanu G, Szedlacsek SEIonescu AE et al . "Analysis of EYA3 Phosphorylation by Src Kinase Identifies Residues Involved in Cell Proliferation", International Journal of Molecular Sciences 20(24): 6307, (2019)
    IF: 4.18
  • Scheidig AJ, Horvath D, Szedlacsek SE.Scheidig AJ et al . "Crystal structure of a xylulose 5-phosphate phosphoketolase. Insights into the substrate specificity for xylulose 5-phosphate", Journal of Structural Biology 207(1): 85-102, (2019)
    IF: 3.75
  • Rosca AM, Mitroi DN, Cismasiu V, Badea R, Necula-Petrareanu G, Preda MB, Niculite C, Tutuianu R, Szedlacsek S, Burlacu ARosca AM et al . "Collagen regulates the ability of endothelial progenitor cells to protect hypoxic myocardium through a mechanism involving miR-377/VE-PTP axis", Journal of cellular and molecular medicine 22(10): 4700-4708, (2018)
    IF: 4.66
  • Mentel M, Ionescu AE, Puscalau-Girtu I, Helm MS, Badea RA, Rizzoli SO, Szedlacsek SEMentel M et al . "WDR1 is a novel EYA3 substrate and its dephosphorylation induces modifications of the cellular actin cytoskeleton", Scientific reports 8(1): 2910, (2018)
  • Mentel M, Badea RA, Necula-Petrareanu G, Mallikarjuna ST, Ionescu AE, Szedlacsek SEMentel M et al . "Expression, Purification, and Kinetic Analysis of PTP Domains", Methods in molecular biology (Clifton, N.J.) 1447: 39-66, (2016)
  • Petrareanu G, Balasu MC, Vacaru AM, Munteanu CVA, Ionescu AE, Matei I, Szedlacsek SEPetrareanu G et al . "Phosphoketolases from Lactococcus lactis, Leuconostoc mesenteroides and Pseudomonas aeruginosa: Dissimilar sequences, similar substrates but distinct enzymatic characteristics", Applied Microbiology and Biotechnology 18(98): 7855-67, (2014)
    IF: 3.34
  • Pascaru M, Tanase C, Vacaru AM, Boeti P, Neagu E, Popescu I, Szedlacsek SEPascaru M et al . "Analysis of molecular determinants of PRL-3", Journal of cellular and molecular medicine 13(9B): 3141-50, (2009)
    AI: 1.70
  • Mihaela Mentel, Rodica A. Badea, Georgiana Necula - Petrareanu, Sujay T. Mallikarjuna, Aura E. Ionescu, Stefan E. SzedlacsekMihaela Mentel et al . "Expression, Purification, and Kinetic Analysis of PTP Domains", pp 39- 66, Protein Tyrosine Phosphatases, Rafael Pulido, (2016).
    ISBN: 978-1-4939-3744-8
  • Stefan Szedlacsek. "Time-Dependent or Steady-State Control of Metabolic Systems?", pp 251-258, Technological and Medical Implications of Metabolic Control Analysis, A.Cornish-Bowden and M.L.Cardenas, NATO Science Series, 3.High Technology, (2000).
    ISBN: 978-94-011-4072-0
  • Stefan Szedlacsek. "Kinetics of slow and tight-binding inhibitors", pp 144-180, Fundamentals of Enzyme Kinetics, Revised edition by Athel Cornish-Bowden, ACADEMIC PRESS INC, 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495, (1995).
    ISBN: 1855780720
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Grants

Compound for inhibition of certain signaling processes related to the evolution of the cognitive processes 2017-2018
Acronym: Ctr.327/27.03.2017/Company CRU SRL, Medical Services Company/ Dunakesz, Hungary
Budget: 70.000 euro
Project director: Stefan Szedlacsek

Molecular modeling of a set of peptides that can disrupt the GluA2-Cterm complex with STEP (Complex A) or BRAG2 (ComplexB).

Compound for inhibition of certain signaling processes related to the evolution of the cognitive processes
Novel radiolabeled affibodies for targeted imaging and therapy 2019-2022
Acronym: Bilateral agreement no.3698/13.09.2018 Romanian Academy- Hungarian Academy of Sciences
Project director: Stefan Szedlacsek

The project is agreed as a joint collaboration among IBAR and ATOMKI, UD and IFIN-HH is a cost free participant. There are two main directions envisaged by the proposed project: receptors mapping and therapy, using an affibody against HER2 receptor, combined with an adequate radioisotope. In this respect, the specific objectives are: a) expression and purification of affibodies; b) establish labeling procedures; c) ex vivo and/or in vivo testing of optimal compounds.

Structure, Regulation, and Biological Function 2007-2011
Acronym: PTPNET
Budget: 145.231,18 euro
Project director: Stefan Szedlacsek

Enzymatic characterization of both mEya3 and hEya1 to identify the similarities and differences between these two proteins with relatively low sequence homology.

Structure, Regulation, and Biological Function
“Reconstruction of Ancestor of Receptor Protein Tyrosine Phosphatase Catalytic” 2011-2016
Acronym: PN-II-ID-PCE-2011-3-0743
Budget: 1499990
Project director: Stefan Szedlacsek

Synthesis of the catalytic domain of ancestral PTP and its characterization both in vitro and in vivo.