Radiolabelling of affibody for tumor diagnostic and theranostic application in the nuclear medicine

Radiolabelling of affibody for tumor diagnostic and theranostic application in the nuclear medicine 2022-2024
Acronym: Bilateral agreement no.2886/15.09.2021 Romanian Academy- Hungarian Academy of Sciences
Project director: Stefan Szedlacsek
External website: full link

The project is agreed as a joint collaboration among IBAR, ATOMKI and UD, the latter being a cost free participant. There are two main directions envisaged by the proposed project: - receptors mapping and therapy, using an affibody against HER2 receptors, combined with an adequate radioisotope. In this respect, the specific objectives are: a) expression and purification of affibodies; b) establishing labelling procedures; c) ex vivo and/or in vivo testing of optimal compounds.

Stefan Szedlacsek, Dr.
Stefan Szedlacsek, Dr.

Head of Department

Professor Dr. Stefan Eugen Szedlacsek is the Head of the Enzymology Department at the Institute of Biochemistry of the Romanian Academy. He holds a PhD degree in Biotechnology from Polytechnic University of Bucharest as well as a MSc in Organic Synthesis (Polytechnic University- Bucharest) and MSc in Mathematics (Bucharest University). As a visiting scientist, he performed research in the field of cholesterol metabolism University of Illinois at Urbana-Champaign (USA), where he succeeded to evidence a new pathway in the metabolism of oxysterols. He is an “Alexander von Humboldt“ fellow and worked in Germany, in the Institute of Biochemistry (Kiel University) More...

Adina-Gabriela Puiu, PhD Student
Adina-Gabriela Puiu, PhD Student

Research Assistant

Adina-Gabriela Puiu is a researcher in the Institute of Biochemistry of the Romanian Academy. Adina-Gabriela is currently working in Enzymology in the Enzymology.

Andra-Elena Cosoreanu
Andra-Elena Cosoreanu

Andra-Elena Cosoreanu is a researcher in the Institute of Biochemistry of the Romanian Academy. Andra-Elena is currently working in Enzymology in the Enzymology.

Andrei-Mihai Vasilescu, PhD Student
Andrei-Mihai Vasilescu, PhD Student

Research Assistant

Andrei-Mihai Vasilescu is a researcher in the Institute of Biochemistry of the Romanian Academy. Andrei-Mihai is currently working in Enzymology in the Enzymology.

Bica Constantin Catalin, MSc
Bica Constantin Catalin, MSc

Asistent

Bica Constantin Catalin is a researcher in the Institute of Biochemistry of the Romanian Academy. Bica is currently working in Enzymology in the Enzymology.

The Romanian team The Hungarian team

Dr.Stefan Szedlacsek, Romanian Head of Project

PhD Student Adina-Gabriela Puiu

PhD Student Andrei Mihai Vasilescu

PhD Student Andra-Elena Coșoreanu                        

Assistant: Cătălin Constantin Bica

Dr. Szűcs Zoltán, Institute for Nuclear Research  Debrecen,

Hungarian Head of Project

Dr. Gyula Tircsó, University of Debrecen

Dr Károly Brezovcsik, University of Debrecen

Msc. Mátiás Fodor, Institute for Nuclear Research Debrecen

Msc. Balázs Váradi, University of Debrecen

Msc.Alexandra NagyInstitute for Nuclear Research Debrecen

1. The trip of the Hungarian team to Romania between: May 7-14, 2023;

During this first visit, the experiments were aimed at producing and purifyng the HER0-2 affibody, as well as performing optical imaging (OI) experiments with the affibody-PC2A-EA-Tb-conjugated complex in a receptor-affibody reaction with 2 cell lines, SKOV-3 and HEK-293T. Affibody expression was performed with Escherichia Coli.

2. Travel of the Romanian team to Hungary in the period May: 15-24, 2023;

During this period, the Romanian team achieved next activities:

A. Activities carried out within the Atomki Institute: - labeling of the PC2A-EA chelator with radioactive 155/156Tb

B. Activities carried out within the University of Debrecen

- conjugation reaction between anti-HER2 Affibody and Alexa Fluor 555-MMA

- tracking the progress of the conjugation reaction

3The trip of the Hungarian team to Romania between: June 19-23, 2023;

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