Ligand-Receptor Interactions

Ligand-Receptor Interactions Department

Ligand-Receptor Interactions

Lactoferrin (Lf) is an iron binding glycoprotein of transferrin family, present in almost all mammalian secretions and neutrophils. A variety of biological functions have been ascribed to Lf, including anti-inflammatory and immunomodulatory effects. The protective action of Lf during inflammation is related to its influence on cytokine production and capacity to bind potentially toxic iron at inflammation foci. In our previous studies performed in collaboration with the Department of Clinical Bacteriology, University of Goteborg, we found that LPS (lipopolysaccharide) -induced cytokine production, in particular TNF-a, IL-1β, IL-6, are inhibited by Lf. The binding of protein to LPS could partly explain its inhibitory activity since the effect could be seen when Lf was added before as well after cell stimulation. This suggests that other mechanism(s) than simple LPS scavenging property of Lf is (are) responsible for the inhibitory activity.
In this respect, our group approaches two aspects:



The mode of action of Lf on intracellular signal transduction pathways.



Our preliminary results showed that Lf is taken up by monocytic cells. Therefore it is possible that Lf, following the binding to the cells, might affect some  intracellular pathways involved in the cell response to external stimuli such as bacterial endotoxins. LPS stimulation of monocytes  has been shown to induce  the activation  of three subgroups of MAPKinase familly-p38, ERK1/2 and JNK. These enzymes play an important role in the control of proinflammatory cytokine production mediated by LPS. We propose to study the effect of Lf on the phosphorylation  and activation of MAPKinases in the presence/absence of LPS. The results will be related to the effect of Lf on the LPS-induced cytokine production in monocytic THP-1 cells. This aspect involves collaboration with Dr. Inger Mattsby-Baltzer group from the Department of Clinical Bacteriology, University of Gothenburg, Sweden.



The anti-inflammatory properties of Lf in vivo: use of liposomes as possible carriers for Lf in the treatment of Rheumatoid Arthritis.


Exhibiting antimicrobial and anti-inflammatory activities Lf could have therapeutic potential in arthritic disease. We are investigating the ability of free and liposome-entrapped Lf to reduce inflammation when administrated to the joint. Current studies aim to establish the suitable liposomal formulation able to deliver Lf efficiently into the joint of mice with collagen-induced rheumatoid arthritis.Liposomes-entrapped Lf is expected to serve as a better tool and therapeutic agent against inflammation. This part is developed in collaboration with Dr. James Brewer group from the Department of Clinical Immunology, Glasgow University, UK, coordinated by Prof. F. Y. Liew.

The project should provide important information for understanding the anti-inflammatory activity of Lf in vivo.

In the next years our research will focus on the cellular and molecular mechanisms involved in the anti-inflammatory and immunomodulatory activities of Lf.

Anca Roseanu, Dr.
Anca Roseanu, Dr.

Head of Department

TBA More...

Madalina Icriverzi, PhD
Madalina Icriverzi, PhD

Research Assistant

B.Sc. in Biochemistry at Faculty of Biology, University of Bucharest. Master in Biochemistry and Molecular Biology (University of Bucharest); research short internship at University of Gothenburg, Sweden; PhD student at th More...

Magdalena Moisei, PhD
Magdalena Moisei, PhD

senior scientific researcher

Facultatea de Tehnologie Chimica/ Institutul Politehnic Bucuresti Diploma de licenta specializarea Biochimie Doctor in Biologie, specializarea Biochimie Teză de doctorat : “Implicarea glicoconjugatelor de membrană în m More...

Mihaela Trif, PhD
Mihaela Trif, PhD

Senior Scientific Researcher

-BS in Biology/ Biochemistry Section; MSc in Enzymology/ Faculty of Biology, University of Bucharest (1979); -PhD in Biology, Biochemistry field - disertation title „Liposome as carriers for active principle” / Institut More...

Paula Florian, Dr.
Paula Florian, Dr.

Research Scientist

Faculty of Biochemistry More...

Year
  1. Nedelcu I, Florian P, Ion D, Militaru E, Damalan A, Popescu CI, Hristea ANedelcu I et al . "Dynamics of serum cross-neutralization capacity against SARS-CoV-2 Delta variant in convalescent COVID-19 patients", Journal of medical virology 96(2): e29448, (2024)
    doi: 10.1002/jmv.29448
    IF: 6.80AI: 1.82
  2. Madalina Icriverzi, Paula Ecaterina Florian, Anca Bonciu, Luminita Nicoleta Dumitrescu, Antoniu Moldovan, Diana Pelinescu, Robertina Ionescu, Ionela Avram, Cristian VA Munteanu, Livia Elena Sima, Valentina Dinca, Laurentiu Rusen, Anca RoseanuMadalina Icriverzi et al . "Hybrid bio-nanoporous peptide loaded-polymer platforms with anticancer and antibacterial activities", Nanoscale Advances, (2024)
    doi: 10.1039/D3NA00947E
    IF: 4.60AI: 0.86
  3. Nistorescu S, Icriverzi M, Florian PE, Bonciu A, Marascu V, Dumitrescu N, Gradisteanu Pircalabioru G, Rusen L, Mocanu A, Roseanu A, Cimpean A, Grama F, Dinca V, Cristian DANistorescu S et al . "Mitigation of Cellular and Bacterial Adhesion on Laser Modified Poly (2 Methacryloyloxyethyl Phosphorylcholine)/Polydimethylsiloxane Surface", Nanomaterials 13(1): 64, (2023)
    IF: 5.72AI: 0.70
  4. Toderascu LI, Sima LE, Orobeti S, Florian PE, Icriverzi M, Maraloiu V-A, Comanescu C, Iacob N, Kuncser V, Antohe I, Popescu-Pelin G, Stanciu G, Ionita P, Mihailescu CN, Socol GToderascu LI et al . "Synthesis and Anti-Melanoma Activity of L-Cysteine-Coated Iron Oxide Nanoparticles Loaded with Doxorubicin", Nanomaterials 13(4): 621-642, (2023)
    IF: 5.72AI: 0.70
  5. Jipa F, Florian P, Icriverzi M, Popescu-Pelin G Budei D, Axente E, Sugioka K, Sima FJipa F et al . "Titanium Surface Nanostructuring by Picosecond Laser Irradiation for Surface Improvement of Dental Abutments", JLMN-Journal of Laser Micro/Nanoengineering 18(3): 127-132, (2023)
    doi: 10.2961/jlmn.2023.03.2003
    IF: 1.10AI: 0.15
  6. Dumitrescu NL, Icriverzi M, Bonciu A, Florian P, Moldovan A, Roseanu A, Rusen L, Dinca V, Grama FDumitrescu NL et al . "New Poly(N-isopropylacrylamide-butylacrylate) Copolymer Biointerfaces and Their Characteristic Influence on Cell Behavior In Vitro", International journal of molecular sciences 23(7), (2022)
    IF: 5.92AI: 1.12
  7. Gherasim O, Popescu-Pelin G, Florian P, Icriverzi M, Roseanu A, Mitran V, Cimpean A, Socol GGherasim O et al . "Bioactive Ibuprofen-Loaded PLGA Coatings for Multifunctional Surface Modification of Medical Devices", Polymers 13(9), (2021)
    IF: 4.33AI: 0.90
  8. Bonciu AF, Orobeti S, Sima LE, Icriverzi M, Filipescu M, Moldovan A, Popescu A, Dinca V, Dinescu M, Bonciu AF et al . "Pyramidal shaped ceria nano-biointerfaces for studying the early bone cell response", Applied Surface Science 533(10): 147464, (2020)
    IF: 6.70AI: 0.60
  9. Florian PE, Icriverzi M, Ninciuleanu CM, Alexandrescu E, Trica B, Preda S, Ianchis R, Roseanu AFlorian PE et al . "Salecan-Clay Based Polymer Nanocomposites for Chemotherapeutic Drug Delivery Systems; Characterization and In Vitro Biocompatibility Studies", Materials (Basel, Switzerland) 13(23), (2020)
    IF: 3.62AI: 0.80
  10. Icriverzi M, Dinca V, Moisei M, Evans RW, Trif M, Roseanu AIcriverzi M et al . "Lactoferrin in Bone Tissue Regeneration", Current medicinal chemistry 12(16), (2019)
    IF: 3.89
  11. Icriverzi M, Bonciu A, Rusen L, Sima LE, Brajnicov S, Cimpean A, Evans RW, Dinca V, Roseanu AIcriverzi M et al . "Human Mesenchymal Stem Cell Response to Lactoferrin-based Composite Coatings", Materials (Basel, Switzerland) 12(20), (2019)
    IF: 2.97AI: 0.80
  12. Grumezescu V, Gherasim O, Negut I, Banita S, Holban AM, Florian P, Icriverzi M, Socol GGrumezescu V et al . "Nanomagnetite-embedded PLGA Spheres for Multipurpose Medical Applications", Materials (Basel, Switzerland) 12(16), (2019)
    IF: 2.97
  13. Florian P, Duta L, Grumezescu V, Popescu-Pelin G, Popescu A, Oktar F, Evans R, Roseanu Constantinescu A.Florian P et al . "Lithium-Doped Biological-Derived Hydroxyapatite Coatings Sustain In Vitro Differentiation of Human Primary Mesenchymal Stem Cells to Osteoblasts", Coatings 12(9), (2019)
    IF: 2.35
  14. Icriverzi M, Rusen L, Brajnicov S, Bonciu A, Dinescu M, Cimpean A, Evans RW, Dinca V, Roseanu AIcriverzi M et al . "Macrophage in vitro Response on Hybrid Coatings Obtained by Matrix Assisted Pulsed Laser Evaporation", Coatings(9), (2019)
    IF: 2.35
  15. M. Icriverzi, L. Rusen, L. E. Sima, A. Moldovan, S. Brajnicov, A. Bonciu, N. Mihailescu, M. Dinescu, A. Cimpean, A. Roseanu, V. DincaM. Icriverzi et al . "In vitro behavior of human mesenchymal stem cells on poly(N-isopropylacrylamide) based biointerfaces obtained by matrix assisted pulsed laser evaporation", Applied Surface Science 440: 712-724, (2018)
    IF: 5.16AI: 0.60
  16. Popescu A C, Florian P E, Stan G E, Popescu-Pelin G, Zgura I, Enculescu M, Oktar F N, Trusca R, Sima L E, Roseanu A, Duta LPopescu A C et al . "Physical-chemical characterization and biological assessment of simple and lithium-doped biological-derived hydroxyapatite thin films for a new generation of metallic implants", Applied Surface Science 439: 724-735, (2018)
    IF: 5.15AI: 0.60
  17. Dinca V, Mocanu A, Isopencu G, Busuioc C, Brajnicov S, Vlad A, Icriverzi M, Roseanu A, Dinescu M, Stroescu M.Dinca V et al . "Biocompatible pure ZnO nanoparticles-3D bacterial cellulose biointerfaces with antibacterial properties", Arabian Journal of Chemistry, (2018)
    IF: 2.96
  18. Popescu A., Florian P., Stan G., Popescu-Pelin G., Zgura I., Enculescu M., Oktar F., Trusca R., Sima L., Roseanu APopescu A. et al . "Physical-chemical characterization and biological assessment of simple and lithium-doped biological-derived hydroxyapatite thin films for a new generation of metallic implants", (Applied Surface Science), (2018)
  19. Clatici VG, Voicu C, Voaides C, Roseanu A, Icriverzi M, Jurcoane SClatici VG et al . "Diseases of Civilization - Cancer, Diabetes, Obesity and Acne - the Implication of Milk, IGF-1 and mTORC1", Maedica 13(4): 273-281, (2018)
  20. Scarisoreanu ND, Craciun F, Ion V, Birjega R, Bercea A, Dinca V, Dinescu M, Sima LE, Icriverzi M, Roseanu A, Gruionu L, Gruionu GScarisoreanu ND et al . "Lead-Free Piezoelectric (Ba,Ca)(Zr,Ti)O3 Thin Films for Biocompatible and Flexible Devices", ACS applied materials & interfaces 9(1): 266-278, (2017)
    IF: 8.30AI: 1.50
  21. Moisei Magdalena, Craciunescu Oana, Moldovan Lucia, Roseanu Anca, Trif MihaelaMoisei Magdalena et al . "Lipid Nanostructures Containing Atorvastatin Decrease Lipopolysaccharide-Induced Inflammation in Macrophages", (Journal of Nanoscience and Nanotechnology), (2017)
  22. Rusen, L.; Dinca, V.; Mustaciosu, C.; Icriverzi, M.; Sima, L.E.; Bonciu, A.; Brajnicov, S.; Mihailescu, N.; Dumitrescu, N.; Popovici, A.I., Roseanu A.; Dinescu MRusen et al . "Modern Technologies for Creating the Thin-Film Systems and Coatings", : 171-191, (2017)
  23. Ianchis R, Ninciuleanu CM, Gifu IC, Alexandrescu E, Somoghi R, Gabor AR, Preda S, Nistor CL, Nitu S, Petcu C, Icriverzi M, Florian PE, Roseanu AMIanchis R et al . "Novel Hydrogel-Advanced Modified Clay Nanocomposites as Possible Vehicles for Drug Delivery and Controlled Release", Nanomaterials (Basel, Switzerland) 7(12), (2017)
  24. Trif M and Craciunescu O. "Liposomes as Delivery System of Chondroitin Sulfate to the Arthritic Joint by Intra-articular Administration", Austin Arthritis 1(3): 1011, (2016)
  25. Paula Ecaterina Florian, Georgiana Petrareanu, Simona Maria Ruţă, Anca RoseanuPaula Ecaterina Florian et al . "Optimization of Recombinant Lipoic Acid Ligase Expression from Bacterial Cells", (Romanian Biotechnological Letters), (2016)
  26. Florian PE, Rouillé Y, Ruta S, Nichita N, Roseanu AFlorian PE et al . "Recent advances in human viruses imaging studies", Journal of basic microbiology 56(6): 591-607, (2016)
  27. Trif M, Florian PE, Roseanu A, Moisei M, Craciunescu O, Astete CE, Sabliov CMTrif M et al . "Cytotoxicity and intracellular fate of PLGA and chitosan-coated PLGA nanoparticles in Madin-Darby bovine kidney (MDBK) and human colorectal adenocarcinoma (Colo 205) cells", Journal of biomedical materials research. Part A 103(11): 3599-611, (2015)
    IF: 3.26
  28. Macovei A, Petrareanu C, Lazar C, Florian P, Branza-Nichita NMacovei A et al . "Regulation of hepatitis B virus infection by Rab5, Rab7, and the endolysosomal compartment", Journal of virology 87(11): 6415-27, (2013)
    IF: 4.65AI: 1.50
  29. Craciunescu O, Moldovan L, Moisei M, Trif MCraciunescu O et al . "Liposomal formulation of chondroitin sulfate enhances its antioxidant and anti-inflammatory potential in L929 fibroblast cell line", Journal of liposome research 23(2): 145-53, (2013)
  30. Roseanu A, Florian PE, Moisei M, Sima LE, Evans RW, Trif MRoseanu A et al . "Liposomalization of lactoferrin enhanced its anti-tumoral effects on melanoma cells", Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine 23(3): 485-92, (2010)
  31. Constantinescu C, Palla-Papavlu A, Rotaru A, Florian P, Chelu F, Icriverzi M, Nedelcea A, Dinca V, Roseanu A, Dinescu M.Constantinescu C et al . "Multifunctional thin films of lactoferrin for biochemical use deposited by MAPLE technique", Applied Surface Science 255(10): 5491-5495, (2009)
    AI: 0.60
  32. Trif M, Roseanu A, Brock JH, Brewer JMTrif M et al . "Designing lipid nanostructures for local delivery of biologically active macromolecules", Journal of liposome research 17(3-4): 237-48, (2007)
    AI: 0.30
  33. Raducanu A, Stoica C, Icriverzi M, Lazar C, Nichita N, Roseanu A.Raducanu A et al . "Antiviral activity of lactoferrin against bovine viral diarrhea virus", Romanian Jornal of Biochemistry(42): 21-29, (2005)
  34. Trif M, Guillen C, Vaughan DM, Telfer JM, Brewer JM, Roseanu A, Brock JHTrif M et al . "Liposomes as possible carriers for lactoferrin in the local treatment of inflammatory diseases", Experimental biology and medicine (Maywood, N.J.) 226(6): 559-64, (2001)
Intarirea capacitatii administrative a Institutului de Biochimie 2010
Acronym: ICAIB
Project director: Anca Roseanu

Începând cu data de 16.06.2010, Institutul de Biochimie beneficiază de asistenţă financiară nerambursabilă pentru implementarea proiectului “Întărirea capacităţii administrative”, în baza contractului de finanţare nr. 167/16.06.2010 semnat cu Organismul Intermediar ANCS Bucureşti.